#10x chromium read length free

Single cell RNA sequencing of mock and SARS-CoV-2 infected Vero E6 cells performed with either 10X Genomics Chromium Next GEM Single Cell 3' (10X 3') or 10X Genomics Chromium Next GEM Single Cell V(D)J (10X 5'). Mock and infected samples were loaded on separate lanes of a 10X Genomics Chromium Controller, according to manufacturer's protocol, for either NextGem Single Cell 3' v3.1 (10X 3') or NextGEM Single Cell V(D)J v1.1 (10X 5'). Cells were then filtered through a 40μm FlowMi strainer (ScienceWare # H13680-0040) and counted again.

#10x chromium read length free

Cells were then centrifuged (200 x g for 5 minutes), resuspended in calcium/magnesium free PBS supplemented with 1% BSA and counted. Vero E6 cells were cultured with Dulbecco's Modified Eagle Medium (DMEM) with 10% fetal bovine serum (FBS) and 1% penicillin-streptomycin (PSN) at 37☌ and 5% CO2.Ĭultures were washed with calcium/magnesium-PBS and disassociated with TrypLE (Gibco # 12605010) for 5 minutes at 37☌. In 6 well plates, cells were infected with SARS-CoV-2 (USA-WA1/2020) at an MOI of 0.1 or mock infected with equivalent control media in reduced serum media (2% FBS, 1% PSN) for 24 hours. Sequencing method: Extended R1 Protocol (Read 1 158 nt, i7 index 8 nt, no read 2) Scrnaseq method: 10X Genomics Chromium Next GEM Single Cell V(D)J v1.1 Vero E6 African Green Monkey kidney cell lineĬell line: Vero E6 African Green Monkey kidney cell line GEO help: Mouse over screen elements for information.